School of Dentistry.
Faculty of Medicine.
P. U. Católica de Chile.
1992. Faculty of Dentistry, University of Chile. Specialty in Periodontics.
1997. Faculty of Dentistry, University of Chile. PhD in Biomedical Sciences.
2005. Faculty of Medicine, University of Chile Diploma in Medical Education.
2010. Faculty of Medicine, Pontificia Universidad Católica de Chile.
Regulation of inflammation and wound healing in the periodontium. Periodontal tissues are permanently exposed to aggressions that include colonization and eventual microbial infection and mechanical damage caused by traumatic or surgical wounds. The reparative response is fundamental to recover the homeostasis and eventual regeneration of the tissue after the damage of these structures. Unfortunately, tissue healing and regeneration does not always occur and very often a deficient reparative response can result in poor wound closure at the epithelial level, an abnormal reconstitution of connective tissue, loss or destruction of bone tissue and maintenance of inflammation. Through multiple studies we have evaluated how different conditions such as smoking, diabetes and ultimately aging can negatively modulate tissue repair in the periodontium. For this we have focused especially on the role played by connective tissue fibroblasts and their differentiation into myofibroblasts. These myofibroblasts play a critical role in the regeneration of tissue through the synthesis of collagen and the organization of a functional extracellular matrix. Our studies point out that deficiencies in the differentiation and / or functionality of these cells are responsible for defects in tissue repair associated with diabetes and / or aging. These studies involve different experimental approaches that include primary cultures of gingival connective tissue cells, pre-clinical models of inflammation and wound repair, and analysis of tissues of human origin.
ISI Publications During the Last 5 Years:
Smith PC. Role of myofibroblasts in normal and pathological periodontal wound healing. Oral Diseases. In Press. [IF: 2.011]
Saldías MP, Fernández C, Morgan A, Díaz C, Morales D, Jaña F, Gómez A, Silva A, Briceño F, Oyarzún A, Maldonado F, Cerda O, Smith PC, Cáceres M. Aged blood factors decrease cellular responses associated with delayed gingival wound repair. PLoS One. 2017 Sep 12;12(9):e0184189. doi: 10.1371/journal.pone.0184189. eCollection 2017. [IF: 3.54]
Pavez L, Tobar N, Chacón C, Arancibia R, Martínez C, Tapia C, Pastor A, González M, Martínez J, Smith PC. Chitosan-Triclosan Particles Modulate Inflammatory Signaling In Gingival Fibroblasts. Journal of Periodontal Research. In Press. [IF: 2.662]
Retamal IN, Hernández R, Melo F, Zapata P, Martínez C, Martínez J, Smith PC. Glycated Collagen Stimulates Differentiation of Gingival Myofibroblasts. J Periodontol. 2017; 88: 926-935. doi: 10.1902/jop.2017.160730. [IF: 2.84]
Lopez R, Smith PC, Gostemeyer G, Schwendicke F. Ageing, dental caries and periodontal diseases. J Clin Periodontol 2017; 44 (Suppl. 18): S145–S152. doi: 10. 1111/jcpe.12683. [IF: 3.477]
Tobar N, Porras O, Smith PC, Barros LF, Martínez J. Modulation of Mammary Stromal Cell Lactate Dynamics by Ambient Glucose and Epithelial Factors. J Cell Physiol. 2017 Jan; 232(1): 136-144. doi: 10.1002/jcp.25398. [IF: 4.08]
Kallens V, Tobar N, Molina J, Bidegain A, Smith PC, Porras O, Martínez J. Glucose Promotes a Pro-Oxidant and Pro-Inflammatory Stromal Microenvironment which Favors Motile Properties in Breast Tumor Cells. J Cell Biochem. 2017; 118: 994-1002. doi: 10.1002/jcb.25650. [IF: 3.085]
Retamal IN, Hernández R, González-Rivas C, Cáceres C, Arancibia R, Romero A, Martínez C, Tobar N, Martínez J, Smith PC. Methylglyoxal and methylglyoxal-modified collagen as inducers of cellular injury in gingival connective tissue cells. J Periodontal Res. 2016; 51: 812-821. doi: 10.1111/jre.12365. [IF: 2.662]
Martinez C, Gonzalez S, Palma V, Smith PC. Platelet Poor Plasma and Platelet Rich Plasma Stimulate Bone Lineage Differentiation in Periodontal Ligament Stem Cells. J Periodontol 2016; 87(2):e18-26. doi: 10.1902/jop.2015.150360. [IF: 2.84]
Martínez C, Smith P, Palma V. The influence of platelet- derived products on angiogenesis and tissue repair: a concise update. Frontiers in Physiology 2015 Oct 20;6:290. doi: 10.3389/fphys.2015.00290. eCollection 2015. [IF: 4.134]
Smith PC, Martínez C, Cáceres M, Martínez J. Research on growth factors in periodontology. Periodontol 2000. 2015; 67: 234-250. doi: 10.1111/prd.12068. [IF: 4.072]
Smith PC, Cáceres M, Martínez C, Oyarzún A, Martínez J. Gingival Wound Healing: An Essential Response Disturbed by Aging? J Dent Res. 2015; 94: 395-402. [IF: 4.755]
Romero A, Cáceres M, Arancibia R, Silva D, Couve E, Martínez C, Martínez J, Smith PC. Cigarette smoke condensate inhibits collagen gel contraction and prostaglandin E2 production in human gingival fibroblasts. J Periodontal Res. 2015; 50: 371-379. doi: 10.1111/jre.12216. [IF: 2.662]
Tobar N, Toyos M, Urra C, Méndez N, Arancibia R, Smith PC, Martínez J. C-Jun N terminal kinase modulates NOX-4 derived ROS production and myofibroblasts differentiation in human breast stromal cells. BMC Cancer. 2014; 14: 640. [IF: 3.288]
Cáceres M, Oyarzún A, Smith PC. Defective wound healing in the aging gingival tissue. J Dent Res 2014; 93: 691-697. [IF: 4.755]
Tobar N, Avalos MC, Méndez N, Smith PC, Bernabeu C, Quintanilla M, Martínez J. Soluble MMP-14 produced by bone marrow-derived stromal cells sheds epithelial endoglin modulating the migratory properties of human breast cancer cells. Carcinogenesis 2014; 35: 1770-1779. doi: 10.1093/carcin/bgu061. [IF: 5.105]
Inzunza D, Covarrubias C, Von Marttens A, Leighton Y, Carvajal JC, Valenzuela F, Díaz-Dosque M, Méndez N, Martínez C, Pino AM, Rodríguez JP, Cáceres M, Smith P. Synthesis of nanostructured porous silica coatings on titanium and their cell adhesive and osteogenic differentiation properties. J Biomed Mater Res A. 2014; 102: 37-48. doi: 10.1002/jbm.a.34673. [IF: 3.189]
Arancibia R, Maturana C, Silva D, Tobar N, Tapia C, Salazar JC, Martínez J, Smith PC. Effects of chitosan particles in periodontal pathogens and gingival fibroblasts. J Dent Res. 2013: 92: 740-745. [IF: 4.755]
Silva D, Arancibia R, Tapia C, Acuña-Rougier C, Diaz-Dosque M, Cáceres M, Martínez J, Smith PC. Chitosan and Platelet-derived growth factor synergistically stimulate cell proliferation in gingival fibroblasts. J Periodontal Res. 2013 48: 677-686. doi: 10.1111/jre.12053. [IF: 2.662]
Arancibia R, Oyarzún A, Silva D, Tobar N, Martínez J, Smith PC. TNF-alpha inhibits TGF-beta-stimulated myofibroblastic differentiation and extracellular matrix production in human gingival fibroblasts. J Periodontol. 2013; 84: 683-693. [IF: 2.84]
Valenzuela F, Covarrubias C, Diaz-Dosque M, Smith P, Martínez C, Yazdani-Pedram M. Preparation and bioactive properties of novel bone-repair bionanocomposites based on hydroxyapatite and bioactive glass nanoparticles. Journal of Biomedical Materials Research: Part B – Applied Biomaterials. 2012; 100: 1672-1682. [IF: 3.189]
Silva D, Cáceres M, Arancibia R, Martínez C, Martínez J, Smith PC. Effects of cigarette smoke and nicotine on cell viability, migration and myofibroblastic differentiation. J Periodontal Res. 2012; 47: 559-607. [IF: 2.662]
Cáceres M, Martínez C, Martínez J, Smith PC. Effects of Platelet Rich and Poor Plasma on the reparative response of gingival fibroblasts. Clin Oral Implant Res 2012; 23: 1104-1111. [IF: 3.624]
Cáceres M, Romero A, Copaja M, Díaz-Araya G, Martínez J, Smith PC. Simvastatin alters fibroblastic cell responses involved in tissue repair. J Periodontal Res 2011; 46: 456–463. [IF: 2.662]
Martínez C, Smith PC, Rodriguez JP. Palma P. Sonic Hedgehog stimulates Proliferation of Human Ligament Periodontal Stem Cells. J Dent Res 2011; 90: 483-488. [IF: 4.755]
Research Grants (Last 10 years):
Gingival wound healing and aging: Role of cellular senescence and the transcription factor FOXO1. FONDECYT 1170555. Principal Investigator: Patricio Smith.
2013- 2016 Diabetes como un modulador de la respuesta de fibroblastos durante la reparación de heridas periodontales. FONDECYT 1130618. Principal Investigator: Patricio Smith.
2013-2015 Effect of the Platelet Rich Plasma and Platelet Poor Plasma on self-renewal, proliferation and differentiation of Human Periodontal Ligament Stem Cells. FONDECYT 11121294. Principal Investigator: Constanza Martínez. Co-investigator: Patricio Smith.
2012-2014 Aging as a modulator of tissue regeneration and inflammation in the periodontium. Postdoctoral grant from Mónica Cáceres (PI) FONDECYT 3120041. Advisor: Patricio Smith. 2010- 2012 Development of a dental product for the prevention and treatment of periodontal diseases. Proyecto FONDEF I + D. DO8I1141. Principal Investigator: Patricio Smith.
2009- 2012 Repair of human periodontal tissue. Modulation by tobacco smoking and growth factors. FONDECYT 1090142. Principal Investigator: Patricio Smith 2006-2008 Regulatory mechanisms involved in tissue remodeling in periodontal diseases. FONDECYT Regular 1061065. Investigador Responsable: Patricio Smith.